Composite
Part:BBa_K3396016
Designed by: Changtai Xiao Group: iGEM20_NUDT_CHINA (2020-10-26)
Revision as of 18:30, 26 October 2020 by Qiuxinyuan12 (Talk | contribs)
CMV-Replaceable-1 -Fluc-P2A-Rluc
A dual luciferase reporter to monitor the Fluc tagged protein abundance while rluc can be used to normalize irrelevant factors.
Usage and Biology
BBa_K3396008 is a Dual Luciferase Reporter system to quantify the abundance of specific target protein. Dual luciferase assay was introduced to normalize the differences caused by irrelevant factors.
Special Design
Special designs were taken to optimize the applicability and adaptivity of such parts. Specifically, a novel designed substitution system, through which, target proteins could be fused with Fluc fragment using Golden-Gate Assembly, was introduced to dramatically simplify the cloning process.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2813
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3482
Illegal BamHI site found at 4020 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2770
Illegal NgoMIV site found at 4387
Illegal NgoMIV site found at 5731
Illegal NgoMIV site found at 5752
Illegal AgeI site found at 1615
Illegal AgeI site found at 5455 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 721
Illegal BsaI site found at 2125
Illegal BsaI site found at 3860
Illegal BsaI.rc site found at 6618
Illegal SapI.rc site found at 2473
Illegal SapI.rc site found at 5637
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Categories
Parameters
None |