Part:BBa_K3525001

T7 pro-His-ER sensor-His-T7 ter

BBa_K3525001 contains BBa_K3525000, a sensor, used to detect endocrine-disrupting chemicals (EDCs).

Contribution

Composite part BBa_K3525001 contains ER-sensor-S protein coding sequence. It is used to express and purify ER-sensor-S protein. We have carried out molecular biology experiments in a laboratory, and have successfully constructed the designed ER-sensor-S protein coding sequence into the expression vector pET28a by PCR and multi-fragment assembly reaction.

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Engineering Success

We plan to test the functionality of our constructed ER Senor in presence of estrogen. To accomplish this functional test, we made the following experiments: 1. The plasmid pET28a-ER-sensor was transferred into BL21 (DE3) and induced by IPTG. We tested and verified the expression of ER sensor protein by SDS-PAGE.

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Figure 1: Expression and characterization of ER sensor protein 1: Negative control, 2: Lysate of ER sensor protein expression strain, 3: Estrogen molecules added into the lysate of ER sensor protein expression strain, 4: Positive control of LacZ expression bacteria lysate