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Part:BBa_K3562015
CCL8
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Chemokines are a family of small chemotactic cytokines, and the name is derived from their ability to induce directed chemotaxis in nearby responsive cells. All chemokines possess a number of conserved cysteine residues involved in intramolecular disulfide bond formation. Some chemokines can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of cells during normal processes of tissue maintenance or development. Chemokines are found in all vertebrates, some viruses and some bacteria.
CCL8 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
Results
We used the Transwell experiment(chamber diameter: 6.5 mm, Pore diameter of PC membrane: 8 μm, 48 pores/board) combined with flow cytometry to verify the chemotactic ability of this chemokine on the THP1 cell line.(Fig.1)
Besides, we also tested the activity of six other chemokines.(Fig.2)
Reference
[1]Chemokine activity definition
[2]UniProtKB - P80075 (CCL8_HUMAN)
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