Coding

Part:BBa_K3624002:Design

Designed by: Emily Hagens   Group: iGEM20_Lethbridge   (2020-10-25)
Revision as of 16:35, 27 October 2020 by Fabian Rohden (Talk | contribs)

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Ah-AMP1: Antifungal anti-microbial peptide.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 178
    Illegal PstI site found at 192
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 178
    Illegal PstI site found at 192
    Illegal NotI site found at 7
    Illegal NotI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 178
    Illegal PstI site found at 192
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was codon-optimized.


Source

Horse chestnuts (Aesculus hippocastanum).

AMP Database ID: AP00436

References

Guizi Ye, Hongyu Wu, Jinjiang Huang, Wei Wang, Kuikui Ge, Guodong Li, Jiang Zhong, Qingshan Huang, LAMP2: a major update of the database linking antimicrobial peptides, Database, Volume 2020, 2020, baaa061, https://doi.org/10.1093/database/baaa061