Coding

Part:BBa_K3573002:Experience

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-11)
Revision as of 00:17, 26 October 2020 by Jchoe21 (Talk | contribs) (→‎Applications of BBa_K3573002)

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Applications of BBa_K3573002

Our team has amplified HRAS using PCR and cloned the gene into an expression vector.

T--Korea HS--RasPCR.jpg

Plates obtained from transformation

T--Korea HS--RASPlates.jpg


We have tested protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations). After expression, Ni-NTA column was used for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS).

T--Korea HS--RasNiNTA.jpg

User Reviews

UNIQ106597356e404d9a-partinfo-00000000-QINU UNIQ106597356e404d9a-partinfo-00000001-QINU