Coding

Part:BBa_K3573002:Experience

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-11)
Revision as of 00:14, 26 October 2020 by Jchoe21 (Talk | contribs) (→‎Applications of BBa_K3573002)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3573002

Our team has amplified HRAS using PCR and cloned the gene into an expression vector.

T--Korea HS--RasPCR.jpg

500px


We have tested protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations). After expression, Ni-NTA column was used for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS).

T--Korea HS--RasNiNTA.jpg

User Reviews

UNIQ6fcec537bff2c0f7-partinfo-00000000-QINU UNIQ6fcec537bff2c0f7-partinfo-00000001-QINU