Composite

Part:BBa_K3573001:Experience

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-10)
Revision as of 00:00, 26 October 2020 by Jchoe21 (Talk | contribs) (→‎Applications of BBa_K3573001)


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Applications of BBa_K3573001

Our team has amplified scFv(RAS) using PCR and cloned the gene into an expression vector.

T--Korea HS--scFvPCR.jpg


We have tested protein expression in 4 different conditions (2 temperatures X 2 IPTG concentrations). After expression, Ni-NTA column was used for protein purification using the His6-tag located at the N-terminus of CPP-scFv(RAS).

T--Korea HS--scFvNiNTA.jpg


We have confirmed the identity of the protein using Western blot analysis using anti-His6 antibody.

T--Korea HS--scFvWestern.jpg


HRas(G12V) that is described part number BBa_K3573002 was used for binding assay with scFv(RAS) using size-exclusion chromatography.

T--Korea HS--scFvRASBinding.jpg

User Reviews

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