Coding

Part:BBa_K3396015

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)
Revision as of 14:52, 25 October 2020 by FloraLiu (Talk | contribs)


Repleceable-3

This is the antibody of target protein with specific restriction sites that could be recognized by AlwI.

Usage and Biology

This part was designed by us to add antibody conveniently. Two restriction sites of AlwI were added to the 5’ and 3’ end of GFPnano, through which, the binding protein of any target gene can be inserted using Golden-Gate Assembly, with no worry about the multiple illegal sites contained in it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None