Composite

Part:BBa_K3425055

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)
Revision as of 00:07, 28 October 2020 by Thomas A (Talk | contribs)

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Constitutive expression construct of Juniper GFP

Asembled using Type IIS iGEM Loop assembly standard from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 478
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 478
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 478
    Illegal BamHI site found at 687
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 478
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 478
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None