Generator

Part:BBa_K3552012

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)
Revision as of 03:54, 26 October 2020 by Akai-B (Talk | contribs)


GmPilA expressed with EHEC similar generator

The complete circuit including a GmPilA and its generator with EHEC genes which is the gene that we mutated. This part is in the part collection where we have 12 genes that code for the base generator of pilA.

The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029

Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4219
    Illegal BglII site found at 6021
    Illegal BamHI site found at 2440
    Illegal BamHI site found at 4544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7508
    Illegal AgeI site found at 3369
    Illegal AgeI site found at 5989
    Illegal AgeI site found at 8277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9283
    Illegal SapI.rc site found at 5625

Characterization

We expressed this complete circuit plasmid in BL21 and verified the function through Western Blot. We also uses the extracted pili to make batteries and measure the voltage and current.

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Categories
Parameters
None