Composite

Part:BBa_K3589207:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)
Revision as of 01:44, 28 October 2020 by Yanniks (Talk | contribs) (→‎Design Notes)


L2 spectinomycin resistance + BaLac_3xHA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal BglII site found at 3966
    Illegal BglII site found at 4925
    Illegal BamHI site found at 4305
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3519
    Illegal PstI site found at 3606
    Illegal PstI site found at 3670
    Illegal PstI site found at 4042
    Illegal PstI site found at 4330
    Illegal PstI site found at 4407
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 6046
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the MoClo standard and codon optimized for C. reinhardtii.

Source

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References