Intermediate

Part:BBa_K133056:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)
Revision as of 00:34, 30 October 2008 by Ancksha (Talk | contribs)

HpaA-LPP-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 877


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).


Source

GeneArt (HpaA and LPP, primers for RGD-Histop annealing)

References