Composite

Part:BBa_K3589203:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)
Revision as of 01:36, 28 October 2020 by Yanniks (Talk | contribs) (→‎Design Notes)


Level 1Mutant BaLac + cCA + SP20-HA-RGS-8His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1617
    Illegal BglII site found at 2576
    Illegal BamHI site found at 1956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
    Illegal NgoMIV site found at 3816
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the MoClo and is codon optimized for C. reinhardtii.

Source

XXXXXX

References