Coding

Part:BBa_K3468070

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 13:57, 27 October 2020 by Dtvnhy (Talk | contribs)

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PETase T270Q

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from T to Q at 270 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds

The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.

Fig.1 T270Q in PyMOL


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Categories
Parameters
None