Part:BBa_K3440000

LuxI-Myc

Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)-DT(BBa_B0015) Will generate LuxI constitutively.

Usage and Biology

Production of LuxI under constitutive promoter. LuxI can produce 3OC6-HSL.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Colony PCR gel for BBa_K3440000

Western blot with A3 as BBa_K3440000

Transformation plate for BBa_K3440000(A)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 698
  Illegal BglII site found at 736
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]