Composite

Part:BBa_K3440004

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 00:26, 25 October 2020 by JuCo (Talk | contribs) (→‎Characterization)


GFP under Prhl

Prhl(BBa_R0071)-RBS(BBa_B0034)-GFP(BBa_E0040)

Usage and Biology

Produces GFP under Prhl, which can be activated by C4-HSL:RhlR.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Transformation plate for BBa_K3440005(E)
Colony PCR gel for BBa_K3440005(E)and BBa_K3440007(F)
Fluorescence measurements for BBa_K34440005 (E)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
Parameters
colorgreen
emission530
excitation483
originGFP from Aequorea victoria (Jellyfish) and Prhl from Pseudomonas aeruginosa