Composite

Part:BBa_K3440005

Designed by: Aditi Kallai, Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 22:52, 24 October 2020 by JuCo (Talk | contribs) (→‎Characterization)


Lux I with myc tag under Promoter prmA activated in the presence of PFOS

Pprma(BBa_K2911000)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Transformation plate for BBa_K3440005(E)
Transformation plate for BBa_K3440005(E)and BBa_K3440007(F)
Fluorescence measurements for BBa_K34440005 (E)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/signalling/sender
Parameters
originprma promoter from Rhodococcus jostii RHA1 and LuxI from Vibrio fischeri
proteinLuxI
tagMyc