Reporter

Part:BBa_K3466002:Design

Designed by: Marry Xuan, Isabela Gonzalez   Group: iGEM20_FDR-HB_Peru   (2020-10-24)
Revision as of 22:03, 24 October 2020 by Isabelagonzalez (Talk | contribs) (→‎Source)


CP -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 293
    Illegal AgeI site found at 395
    Illegal AgeI site found at 1168
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a spacer to test whether or not it serves to enhance our RFP expression rate.


Source

ttgacggctagctcagtcctaggtacagtgctagcgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggcaccggttgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgaccggtatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaataaagaggagaaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

References