Part:BBa_K3332087

pTrc-2 derivative-RBS-ECFP-terminator

We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter

With this part, the pTrc-2 derivative promoter can be tested by observing the fluorescence intensity/OD.

Usage and Biology

This part can be used to test that if the pTrc-2 derivative promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 derivative promoter.

Fig 1. pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015)

Characterization

The agarose gel electrophoresis images are shown as below:

Fig 2. pTrc-2 derivative_E0420_pSB1C3[BBa_K3332087] digested by Xba I and Pst I (about 1018 bp)

Reference

[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 37
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]