Part:BBa_K112019
Lambda phage lysis device
Lysozyme and holin production are driven by pBad, and antiholin is driven by PconC5.
Characterization
We transformed this device in the vector K112950 into the MC1061 strain, and then picked 5 colonies for each device. We grew these cultures to saturation at 37 degrees Celsius in LB media, and then split into eight 1 mL aliquots. A range of concentrations of arabinose was added to these aliquots, with a starting concentration of 1.3E-3 M and the next 6 samples recieving a four-fold dilution of the previous sample, and an equal volume of water added to the last aliquot. The cultures were then incubated at 37 degrees again for 3.5 hours, and the absorbance at 600nm was measured with a Tecan Xfluor4 Safire2 in a Corning Inc. Costar 3603 plate. The data plotted on a log scale is shown below.
A parallel experiment was performed by taking the same saturated culture before induction with arabinose, diluting 100-fold, growing to mid-log(starting OD .22), before inducing with arabinose as above. The data plotted on a log scale is shown below.
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 33
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 33
Illegal NheI site found at 1184
Illegal NheI site found at 2230
Illegal NheI site found at 2253 - 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 33
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 33
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 33
Illegal NgoMIV site found at 2616 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1050
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