Composite
Part:BBa_K3440011
Designed by: Julie Cordier Group: iGEM20_Stockholm (2020-10-23)
GFP under constitutive promoter
Pconst(BBa_J23100)-RBS(BBa_B0034)-GFP(BBa_E0040)-DT(BBa_B0015)
Usage and Biology
Expresses GFP under constitutive promoter
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
[edit]
Categories
Parameters
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//chassis/prokaryote/ecoli
color | green |
emission | 530 |
excitation | 483 |
origin | GFP from Aequorea victoria (Jellyfish) |