Composite
Part:BBa_K3440000
Designed by: Julie Cordier Group: iGEM20_Stockholm (2020-10-23)
LuxI-Myc
Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)-DT(BBa_B0015) Will generate LuxI constitutively.
Usage and Biology
hello
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
Illegal BglII site found at 736 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/ecoli
//classic/signalling/sender
//classic/signalling/sender
origin | Vibrio Fischeri |
protein | LuxI |
tag | Myc |