Composite

Part:BBa_K3373012

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-23)
Revision as of 12:49, 24 October 2020 by Antonin chev (Talk | contribs)


BSR_atzA-HA_atzB-HA_atzC-HA

This part is a multigenenic construct containing three genes involved in the degradation pathway of the pesticide atrazine : atzA, atzB and atzC. All theses genes are under the control of the PSAD promoter and terminator which allow a strong expression in C. reinhardtii. These three CDS are associated with an HA tag allowing immunodetection of the proteins expressed. It also contains a Blasticidin resistance cassette (BSR) encoding for the Blasticidin S Deaminase (BSR) in order to select the recombinant clones after transformation in C. reinhardtii.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal NheI site found at 450
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal XhoI site found at 8887
    Illegal XhoI site found at 8995
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
    Illegal NgoMIV site found at 2418
    Illegal NgoMIV site found at 2818
    Illegal NgoMIV site found at 3659
    Illegal NgoMIV site found at 5136
    Illegal NgoMIV site found at 6173
    Illegal NgoMIV site found at 6588
    Illegal NgoMIV site found at 9283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3725
    Illegal SapI.rc site found at 6654
    Illegal SapI.rc site found at 9349


[edit]
Categories
Parameters
None