Composite

Part:BBa_K3373012:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-23)
Revision as of 12:22, 24 October 2020 by Antonin chev (Talk | contribs) (→‎References)


BSR_atzA-HA_atzB-HA_atzC-HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal NheI site found at 450
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal XhoI site found at 8887
    Illegal XhoI site found at 8995
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1346
    Illegal EcoRI site found at 4254
    Illegal EcoRI site found at 7183
    Illegal PstI site found at 2990
    Illegal PstI site found at 3554
    Illegal NgoMIV site found at 2418
    Illegal NgoMIV site found at 2818
    Illegal NgoMIV site found at 3659
    Illegal NgoMIV site found at 5136
    Illegal NgoMIV site found at 6173
    Illegal NgoMIV site found at 6588
    Illegal NgoMIV site found at 9283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3725
    Illegal SapI.rc site found at 6654
    Illegal SapI.rc site found at 9349


Design Notes

This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriction enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.

Source

The atzA (K3373001); atzB (K3373002) ; atzC (K3373003) enzymes come from the predicted sequence from the Pseudomonas sp. ADP strain genome sequencing.

All the other parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018).

References

1. Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

2. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749.