Composite
Part:BBa_K3519009
Designed by: Omkar Mohapatra Group: iGEM20_IISER-Tirupati_India (2020-10-07)
Revision as of 11:02, 24 October 2020 by Omkarmohapatra123 (Talk | contribs)
medium promoter-traT
This part has the traT gene (BBa_K3519003) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traT gene helps reduce the formation of stable mating pairs. The data provided below are from previous overexpression studies in the literature.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 703
Illegal BsaI.rc site found at 185
[edit]
Categories
Parameters
None |