Coding

Part:BBa_K3429002

Designed by: Jan Lars Kalkowski   Group: iGEM20_TU_Darmstadt   (2020-09-19)
Revision as of 06:04, 25 October 2020 by Mehryad (Talk | contribs)


Superfolder Green Fluorescent Protein (sfGFP)

Flourescence Protein optimized for B. subtilis codon usage.
We use this flexible glycine-serine linker (GGGGSGGGGS) to create TasA fusion proteins with CotA and CuO. Huang et al fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function.



<a class="anchor" id="cite_note-1"></a> 

           <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None