Regulatory

Part:BBa_K3606006

Designed by: Mingwei Li   Group: iGEM20_Fudan   (2020-10-11)
Revision as of 03:44, 25 October 2020 by Cracra (Talk | contribs)


PtetO2 promoter

This part is a fusion promoter of PL and tetO, regulated by tetR and anhydrotetracycline

Design:

This part functions to lower the leakage and raise the level of expression of the downstream gene. As a well-characterized promoter, ptetR has been studied thoroughly with its expression level and leakage. While here we take a unique idea to created a new promoter fusion: we fuse the -35 upstream, -35--10 parts of the stronger pl promoters in lambda phages with the tetO operon regulated by tetracycline. As a promoter fusion of pl and tetO, not only is it regulated by tetR and anhydrotetracycline, but it also inherited the expression level of pl.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None