Composite

Part:BBa_K3328024

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 11:09, 27 October 2020 by Txzz (Talk | contribs) (→‎Result)

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triggers of NIMPLY gate (NIMPLY1)

two input triggers of NIMPLY1 logic gate to perform the truth table

Design

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

799px-T--OUC-China--design_lunbo_nimply.jpg

Result

800px-T--OUC-China--part_NIMPLY1.jpg

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None