Part:BBa_K3599006

afNIT

Overview

Nitrilase coming from Alcaligenes faecalis, catalysis of the reaction: a nitrile + 2H₂O = a carboxylate + NH₃. The codon usage was optimized for expression in Escherichia coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 629
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 67
  
• 1000
  COMPATIBLE WITH RFC[1000]

Primitive Species Background

A genus of gram-negative, rod-shaped, nonfermenting bacteria (family Achromobacteraceae) that are either motile and peritrichous or nonmotile. They are strictly aerobic; some strains are capable of anaerobic respiration in the presence of nitrate or nitrite; their metabolism is respiratory, never fermentative; they do not use carbohydrates. Found mostly in the intestinal canal, decaying materials, dairy products, water, and soil; they can be isolated from human respiratory and gastrointestinal tracts and wounds in hospitalized patients with compromised immune systems; occasionally the cause of opportunistic infections, including nosocomial septicemia.

Design & Experience

In iGEM2020 TPR_China Project, the coding sequence of afNIT were founded and optimized, further characterization of its enzymatic activity could be done by iGEM teams in the fature.

Reference

Liu, Z. Q. , Dong, L. Z. , Cheng, F. , Xue, Y. P. , Wang, Y. S. , & Ding, J. N. , et al. (2011). Gene cloning, expression, and characterization of a nitrilase from alcaligenes faecalis zjutb10. Journal of Agricultural and Food Chemistry. 10.1021/jf202746a