Part:BBa_K3338023

IL-6 P_mut-MagA-P2A-hGLuc

IL-6 P-MagA-P2A-hGLuc is an inflammatory toxin sensor comprising the IL6-Promoter downstream from the MagA-P2A-hGLuc cassette. This part combines the LPS-sensitivity of the IL6-Promoter with the simultaneous expression of the reporter genes MagA for MRI-detection and Gaussia Luciferase for bioluminescence detection in blood or urine (Goldhawk et al. 2009, Zurkiya et al. 2008, Tannous 2009). The construct displays the first step in generating a functional inflammatory toxin sensor for clinical use.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1347
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 220
  Illegal BamHI site found at 395
  Illegal XhoI site found at 1581
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 340
  Illegal BsaI site found at 2803
  Illegal BsaI.rc site found at 387
  Illegal BsaI.rc site found at 696
  Illegal BsaI.rc site found at 2135
  Illegal BsaI.rc site found at 2684
  Illegal SapI site found at 1825
  

References

Goldhawk, D. E., Lemaire, C., McCreary, C. R., McGirr, R., Dhanvantari, S., Thompson, R. T., Figueredo, R., Koropatnick, J., Foster, P., & Prato, F. S. (2009). Magnetic resonance imaging of cells overexpressing MagA, an endogenous contrast agent for live cell imaging. Molecular imaging, 8(3), 129–139.

Tannous B. A. (2009). Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nature protocols, 4(4), 582–591.

Zurkiya, O., Chan, A. W., & Hu, X. (2008). MagA is sufficient for producing magnetic nanoparticles in mammalian cells, making it an MRI reporter. Magnetic resonance in medicine, 59(6), 1225–1231.