Coding

Part:BBa_K3360002:Design

Designed by: Li Wenxi   Group: iGEM20_SZU-China   (2020-10-04)
Revision as of 22:03, 23 October 2020 by Hamlet (Talk | contribs) (→‎Source)


Thermostable beta-glucosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1577
    Illegal AgeI site found at 927
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

Source

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

References