Coding

Part:BBa_K3589109:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)
Revision as of 01:26, 28 October 2020 by Yanniks (Talk | contribs) (→‎Design Notes)


Wildtype laccase from uncultured marine bacteria for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 817
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 817
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 1396
    Illegal NgoMIV site found at 1847
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of Botrytis aclada was removed. This part is codon-optimized for Escherichia coli.

Source

This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Escherichia coli.

References