Part:BBa_K3594013

Heat Responsive Fluorescence Emitting Device

This composite plasmid is used as the reporter of our heat-responsive suicide switch. The expression of sfGFP gene is controlled by an RNA-based thermal sensor, which was constructed by Jilin_China 2018 team.

When the temperature is higher than 37℃, the ribosome starts to work and the downstream gene sequence can be expressed. Specifically, in this part, the fluorescence would be emitted.

Characterization

Each component of bacteria with BBa_K3594013 was divided into 3 biological controls, which were placed at 30℃, 33℃, 37℃, 42℃ with the negative control, positive control and blank control LB, and shaking at 220 rpm. We took samples at 0h, 4h, 8h and 20h respectively, used a microplate reader for spectrophotometer and fluorescence test, and recorded the data in OD600 and Fluorescence 485/510 (Excitation/Emission). We calculated the value of Normalized Fluorescence changes with the equation referenced from Jilin_2018 iGEM team at different temperatures and different time.

File:T--SHSBNU China--the process we do to test the function of BBa K3594013.png

the process we do to test the function of BBa K3594013

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

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• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]