Regulatory

Part:BBa_K3338008:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)
Revision as of 16:29, 23 October 2020 by Jonas Scholz (Talk | contribs) (→‎Design Notes)

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Interleukin-6 Promoter (IL-6 P)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 369
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 369
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696


Design Notes

The sequence is not compatible with any assembly standard. The appropriate IL-6 promoter that shows compatibility is BBa_K3338005.

Source

The plasmid with the sequence of the human IL-6 promoter was purchased from Addgene (Plasmid #79491) (Jäckel et al. 2016).

References

Jäckel, C., Nogueira, M. S., Ehni, N., Kraus, C., Ranke, J., Dohmann, M., Noessner, E., & Nelson, P. J. (2016). A vector platform for the rapid and efficient engineering of stable complex transgenes. Scientific reports, 6, 34365.