Composite

Part:BBa_K3365014:Design

Designed by: JUNYAN LI   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-23)
Revision as of 08:12, 27 October 2020 by Little ghost (Talk | contribs) (→‎Source)


pBAD/araC upstream of RFP with inhibition unit containing target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1913
    Illegal XhoI site found at 1922
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1789
    Illegal AgeI site found at 1901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

target sequence is part of the PDCD1 gene


Source

The ligation of BBa_K3365006, BBa_K3365053 and BBa_K3365002 by Overlap PCR.

References