Coding

Part:BBa_K3493000:Design

Designed by: Emilie Alexander, Benjamin Ouellet   Group: iGEM20_ULaval   (2020-10-22)
Revision as of 00:55, 24 October 2020 by Angelfcc (Talk | contribs)


G. algens dextranase CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for E. coli. Following reports that the Streptococcus mutans homologous dextranase has a higher activity and resistance to proteolysis when its N terminal (residues 1-99) and C-terminal (residues 733-850) are deleted (Kim et al. 2011), we removed them from the CDS submitted to the registry. Accordingly, our final CDS has the contains the region of the G. algens dextranase sequence that aligns to residues 104 - 734 of the PDB structure of the S. mutans dextranase (ref PDB).


Source

The protein sequence is available at NCBI (https://www.ncbi.nlm.nih.gov/protein/WP_066432599.1?report=genbank&log$=prottop&blast_rank=700&RID=MZBBN4NZ014). The gene sequence was inferred from the protein sequence using codon optimization for E. coli.

References