Coding

Part:BBa_K3468064:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 13:48, 27 October 2020 by Dtvnhy (Talk | contribs) (→‎References)

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PETase R260F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. R260F was designed by searching for suitable sites around F261 for mutation.

Source

Ideonalla sakaiensis

References

[1]Burley SK, Petsko GA. Aromatic-aromatic interaction: a mechanism of protein structure stabilization. Science. 1985 Jul 5;229(4708):23-8.

[2]Chatterjee KS, Tripathi V, Das R. A conserved and buried edge-to-face aromatic interaction in small ubiquitin-like modifier (SUMO) has a role in SUMO stability and function. J Biol Chem. 2019 Apr 26;294(17):6772-6784.

[3]Chrtis C, Jaffe R, Maigret B et al. , 1996. Benzene Dimer: A good model for π-π interactions in proteins a comparison between the benzene and the toluene dimers in the gas phase and in an aqueous solution [J] J. Am. Chem. Soc, 118: 11217-11224.

[4]Weber DS, Warren JJ. A survey of methionine-aromatic interaction geometries in the oxidoreductase class of enzymes: What could Met-aromatic interactions be doing near metal sites? J Inorg Biochem. 2018 Sep;186:34-41.