Composite

Part:BBa_K3338011:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)
Revision as of 18:53, 23 October 2020 by Jonas Scholz (Talk | contribs) (→‎Source)

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CMV-EGFP-MagA-P2A-hGLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2553
    Illegal BsaI.rc site found at 1885
    Illegal BsaI.rc site found at 2434
    Illegal SapI site found at 1575


Design Notes

The individual proteins in the EGFP-MagA-P2A-hGLuc-cassette have to be in frame. EGFP was used as a reporter for MagA expression and localization because in order to test transfection efficiency and the correct function of the P2A peptide, MRI detection is to slow and costly.


Source

The CMV-Promoter and EGFP-Reporter were contained in pEGFP-C2. MagA originates from Magnetospirillum magneticum, P2A from the porcine teschovirus 2A and hGLuc is a human codon optimized form of Gaussia luciferase from Gaussia princeps.

References