Coding

Part:BBa_K3589107:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)
Revision as of 01:18, 28 October 2020 by Yanniks (Talk | contribs) (→‎Source)


Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii, Part 1 (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1055
    Illegal BamHI site found at 1394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of the marine bacteria was removed (?). This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.



Source

This part originates from Botrytis aclada (Yang, Q., 2018) and is condon-optimized for Chlamydomonas reinhardtii.

References