Plasmid

Part:BBa_K3505008

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-16)
Revision as of 09:38, 23 October 2020 by Venetios (Talk | contribs)


pDGB1 alpha 1R (α1R) for Bacterial GoldenBraid.

Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening.


Usage and Biology

Fig.1: a1R plasmid Features and Restiction Sites.
Fig.2: a1R Map.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3091
    Illegal PstI site found at 3079
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3118
    Illegal PstI site found at 3079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3118
    Illegal BamHI site found at 11
    Illegal BamHI site found at 2858
    Illegal BamHI site found at 3097
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3091
    Illegal PstI site found at 3079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3091
    Illegal PstI site found at 3079
    Illegal NgoMIV site found at 1290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3461
    Illegal BsaI.rc site found at 2879
[edit]
Categories
//awards/part_collection
//plasmid/construction
//plasmid/expression
Parameters
None