![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3606033:Design
Designed by: Zhenru Zhang Group: iGEM20_Fudan (2020-10-23)
Revision as of 08:41, 25 October 2020 by Zhenru-Zhang (Talk | contribs)
DsbA-CaAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 132
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part DsbA_CaAP is a combination of signal peptide DsbA and CaAP(Calcium Absorption Peptide). The sequence of DsbA is obtained from BBa_K1659002 with no further codon optimization. Then we fused DsbA(BBa_K3606043) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.
Source
The sequence of DsbA is obtained from BBa_K1659002 with no further codon optimization while the sequence of CAAP is artificially designed based on oligopeptides obtained from tilapia bone collagen hydrolysate, etc.