Signalling

Part:BBa_K3365009

Designed by: JUNYAN LI   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 19:10, 27 October 2020 by Little ghost (Talk | contribs)


Lure3 sequence downstream of pBAD

The PAM and potential off-target sequence are located directly downstream of the promoter (BBa_K3365003), where dCas9 might bind wrongly and block RNAP. In our part, the “lure3” is the potential off-target sequence for PDCD1 CRISPR gene editing predicted by our off-target predictor software built in 2019, which might be identified and bound by the complex of dCas9 and sgRNA. According to the off-target predictor software, the relative off-target rate is 73.6673%.

BBa K3365009.png
Figure1. Gene circuit


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


[edit]
Categories
Parameters
None