Coding

Part:BBa_K3562000

Designed by: Haoyu Zheng   Group: iGEM20_WHU-China   (2020-10-22)
Revision as of 15:52, 25 October 2020 by Carotene (Talk | contribs)


BBa_K3562000 short__NOTOC__

BBa_K3562000 short Not understood AHL-lactonase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity. Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2071
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2071
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2071
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2071
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

AHL-lactonase is a kind of lactonase opened the homoserine lactone ring of AHL in the presence of water to produce N-acly homoserine.(Fig.1) The biological activity of degradation products will be greatly reduced.

Figure 1:Mechanism of AHL-lactonase

Quorum sensing factor is closely related to bacterial virulence. Enzymes that degrade AHL can control the concentration of AHL to silence the expression of related genes. In our project, we use these enzymes to control the virulence of Pseudomonas aeruginosa referred to existing experimental therapies.

Different AHL degrading enzymes have different substrate preferences and degradation activities.(Fig.2)

Figure 1:Mechanism of AHL-lactonase

nfo>

AHL-lactonase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2071
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2071
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2071
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2071
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Quorum sensing factor is closely related to bacterial virulence.

Different AHL degrading enzymes have different substrate preferences and degradation activities.

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Parameters
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