Part:BBa_K3335000
We use KIBRA to control exosome secretion
KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a[1].
In our experiment, we used KIBRA to promote cell release of exosomes containing siRNA, thus increasing the efficiency of cell production and release of exosomes and improving the killing of tumor cells.
[1]Lin Song et al,.KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.Nature Communication,2019.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 335
Illegal BglII site found at 2999
Illegal BamHI site found at 246
Illegal BamHI site found at 442 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 435
Illegal BsaI site found at 2598
Illegal BsaI.rc site found at 1920
Illegal BsaI.rc site found at 2644
Functional Parameters
Contribution: NJU-China 2020
This year we want to use liver cells as a concept to treat lung cancer by overexpressing exosomes containing siRNA.
In our interview with the professor, we learned that exosome production is likely to be inefficient, so we found the KIBRA protein.KIBRA as an adaptor - like protein that stabilizes Rab27a, which in turn controls exosome secretion.
Figure 1）qRT-PCR analysis of plasmid-borne ppk1 expression in CPP
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