Part:BBa_K3605004
Endo-β-1,4-glucanase(CMCax) from Gluconacetobacter xylinus.
Endo-β-1,4-glucanase(CMCax) is from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for Bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited.
This part is Endo-β-1,4-glucanase(CMCax) with 1080bp, from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited. This gene was amplified using PCR method, and then inserted into the vector pSB1C3. The identification result is showed in Fig.1.
Fig.1. The result of CMCax gene cloning.
M: Marker; 1: PCR result of CMCax; 2: Digestion of pSB1C3 containing CMCax.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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