Part:BBa_K3605002

Phosphoglucomutase (PGM) and UDP-glucose pyrophosphorylase (UGPase) from Gluconacetobacter xylinus.

This part contains two enzymes (2277bp), which is a polycistron in original organism. One of them is phosphoglucomutase (PGM), it can isomerize the glucose-6-phosphate (G-6-P) to glucose-1-phosphate (G-1-P), which is the second reaction for bacterial cellulose production from glucose. Another enzyme is UDP-glucose pyrophosphorylase (UGPase), it can catalyze the glucose-1-phosphate (G-1-P) to react with UTP, forming uridine-5’-phosphate-α-D-glucose (UDPG), which is the third reaction for Bacterial cellulose production from glucose.

These two enzymes were also amplified using PCR method, and inserted into the vector pSB1C3. Fig.1 shows the identification result.

Fig.1. The result of PGM and UGPase gene cloning. M: Marker; 1: PCR result of PGM and UGPase; 2: Digestion of pSB1C3 containing PGM and UGPase genes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 548
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 478