Reporter

Part:BBa_K3510000

Designed by: team work   Group: iGEM20_Tuebingen   (2020-09-30)
Revision as of 08:03, 21 October 2020 by Famosa (Talk | contribs)

FAST2 reporter gene

This part encodes for the Fluorescence-Activating and Absorption-Shifting Tag (FAST) protein, a 14 kDAsmall protein, that assembles with various fluorgenic ligands (Fluorogens) [1]. Only upon assembly a fluorescent signal is emitted and allows for detection and imaging with high contrast [1].

The original sequence was obtained from the Twinkle Factory and edited in one base pair to eliminate a PstI binding site, so it now follows iGEM’s assembly rules. In our project it was used as a fluorescent tag of the following DNA fragment, either the phytochelatin or chromoprotein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. The Twinkle Factory. The science behind FAST and SplitFAST [cited 2020 Oct 19]. Available from: URL: https://www.the-twinkle-factory.com/the-science-behind-fast-and-splitfast.


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