![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K3512012
Designed by: Gourav Saha Group: iGEM20_BITSPilani-Goa_India (2020-10-20)
Revision as of 05:09, 21 October 2020 by Gourav saha (Talk | contribs)
pFruB-Cra System
A biosensor is a module that combines biological molecules as the recognition element with a physical transducer or repressor and outputs quantitative data corresponding to the biomolecule’s concentration (Park, M et al., 2013). Generally, these specific interactions between the ligand and the transducer are used to determine secretion or production rates in the form of an electrochemical and/or optical signal. In our case, we coupled the biosensor to the expression of anti-invertase.
Mechanism
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2652
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2490
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 170
[edit]
Categories
Parameters
dissociation_constant | Kd = 200nM |