Coding
Part:BBa_K3381001:Design
Designed by: Alina Arvisais Group: iGEM20_Waterloo (2020-10-20)
FutA2 (iron-binding)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design Considerations: The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.
Source
Source of this part: Synechocystis sp. PCC6803, sequence found through GenBank https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=2924779&to=2925819