Part:BBa_K1611000

IFNgamma

Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory.

Part sequencing

Figure 1: Sequencing of IFN gamma cloned in pSB1C3 vector. The black line shows the consensus sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 33
  

MIT_MAHE 2020

Summary

INF-gamma is a cytokine which has been shown to have important roles in tissue homeostasis, immune and inflammatory responses and immunosurveillance of tumor. Its signaling activates the Janus kinase (JAK) which activates transcription 1 (STAT1) pathway. This is expression of classical interferon- stimulated genes that key immune effector functions. Studies have shown effects of IFNγ on other leukocytes, vascular cells, adipose tissue cells, neurons and tumour cells that have important implications for autoimmunity, metabolic diseases, atherosclerosis, neurological diseases and immune checkpoint blockade cancer therapy.

References

1. Ivashkiv L. B. (2018). IFNγ: signalling, epigenetics and roles in immunity, metabolism, disease and cancer immunotherapy. Nature reviews. Immunology, 18(9), 545–558. https://doi.org/10.1038/s41577-018-0029-z